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Displacement chromatography
Displacement chromatography is a chromatography technique in which a sample is placed onto the head of the column and is then displaced by a solute that is more strongly sorbed than the components of the original mixture. The result is that the components are resolved into consecutive “rectangular” zones of highly concentrated pure substances rather than solvent-separated “peaks”.〔N. Tugcu . Purification of proteins using displacement chromatography. pp 71-89 in M. Zachariou (Ed.) Methods in Molecular Biology: Vol 421 Affinity Chromatography: Methods and Protocols. 2nd edition. Humana Press, Totowa NJ.〕 It is primarily a preparative technique; higher product concentration, higher purity, and increased throughput may be obtained compared to other modes of chromatography.
==Discovery==
The advent of displacement chromatography can be attributed to Arne Tiselius,〔A. Tiselius. Displacement development in adsorption analysis. Ark. Kemi. Mineral Geol. 16A: 1–18 (1943).〕 who in 1943 first classified the modes of chromatography as frontal, elution, and displacement. Displacement chromatography found a variety of applications including isolation of transuranic elements 〔G. T. Seaborg. The Transuranium Elements. Science 104(2704):379-386 (1946).
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